The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions

Table 1

Purification table for purification of ChE from P.  javanicus. The specific activity from each step of purification is expressed in (U/mg), which means mole/min/mg of protein.

Procedure	Total protein (mg)	Total ChE activity (U)	Specific activity (U/mg)	Purification folds	Yield (%)
Crude homogenate	296.25	828.13	2.80	1	100
Supernatant (100,000 ×g; 1 h in 4°C)	77.06	688.87	8.94	3.2	83.18
Procainamide-sepharcyl 6B	6.07	263.73	43.44	15.54	38.28