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The Scientific World Journal
Volume 2014, Article ID 571682, 7 pages
http://dx.doi.org/10.1155/2014/571682
Research Article

Optimal Immobilization of β-Galactosidase onto κ-Carrageenan Gel Beads Using Response Surface Methodology and Its Applications

1Polymer Department, National Research Centre, El-Behouth Street, Dokki, Cairo 12311, Egypt
2Centre of Scientific Excellence, Group of Biopolymers and Nanobiotechnology, National Research Centre, El-Behouth Street, Dokki, Cairo 12311, Egypt
3Chemistry Natural & Microbial Products, National Research Centre, El-Behouth Street, Dokki, Cairo 12311, Egypt
4Polymer Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications, Borg Al-Arab, Alexandria 21934, Egypt
5Faculty of Science, Al-Azhar University, Cairo 11651, Egypt

Received 9 August 2013; Accepted 10 November 2013; Published 2 February 2014

Academic Editors: P. Fernandes and X. Zhao

Copyright © 2014 Magdy M. Elnashar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [10 citations]

The following is the list of published articles that have cited the current article.

  • Magdy M. M. Elnashar, and Mohamed E. Hassan, “Novel Epoxy Activated Hydrogels for Solving Lactose Intolerance,” BioMed Research International, vol. 2014, pp. 1–9, 2014. View at Publisher · View at Google Scholar
  • Valeria E. Bosio, Germán A. Islan, Yanina N. Martínez, Nelson Durán, and Guillermo R. Castro, “Nanodevices for the immobilization of therapeutic enzymes,” Critical Reviews in Biotechnology, pp. 1–18, 2015. View at Publisher · View at Google Scholar
  • Marwa I. Wahba, “Treated calcium pectinate beads for the covalent immobilization of β-D-galactosidase,” International Journal of Biological Macromolecules, 2016. View at Publisher · View at Google Scholar
  • Xiaoning Luan, Deqiang Dou, XiaoKu Rana, Mohamed E. Hassan, and Ying Yuan, “Covalent immobilization of cellulase in application of biotransformation of ginsenoside Rb1,” Journal of Molecular Catalysis B: Enzymatic, vol. 133, pp. S525–S532, 2016. View at Publisher · View at Google Scholar
  • Marwa I. Wahba, and Mohamed E. Hassan, “Agar-carrageenan hydrogel blend as a carrier for the covalent immobilization of β-D-galactosidase,” Macromolecular Research, vol. 25, no. 9, pp. 913–923, 2017. View at Publisher · View at Google Scholar
  • Maryam Khan, Qayyum Husain, and Rani Bushra, “Immobilization of β -galactosidase on surface modified cobalt/multiwalled carbon nanotube nanocomposite improves enzyme stability and resistance to inhibitor,” International Journal of Biological Macromolecules, 2017. View at Publisher · View at Google Scholar
  • Ghada E. A. Awad, Hala R. Wehaidy, Abeer A. Abd El Aty, and Mohamed E. Hassan, “A novel alginate–CMC gel beads for efficient covalent inulinase immobilization,” Colloid and Polymer Science, vol. 295, no. 3, pp. 495–506, 2017. View at Publisher · View at Google Scholar
  • Anchal Goel, Rajeshwari Sinha, and Sunil K. Khare, “Immobilization of A. oryzae β-galactosidase on Silica Nanoparticles: Development of an Effective Biosensor for Determination of Lactose in Milk Whey,” Recent advances in Applied Microbiology, pp. 3–18, 2017. View at Publisher · View at Google Scholar
  • Walaa A. Abdel Wahab, Eman A. Karam, Mohamed E. Hassan, Amany L. Kansoh, Mona A. Esawya, and Ghada E.A. Awad, “Optimization of pectinase immobilization on grafted alginate-agar gel beads by 2 4 full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization,” International Journal of Biological Macromolecules, vol. 113, pp. 159–170, 2018. View at Publisher · View at Google Scholar
  • Rukhsana Satar, Mohammad Alam Jafri, Mahmood Rasool, and Shakeel Ahmed Ansari, “Role of Glutaraldehyde in Imparting Stability to Immobilized β-Galactosidase Systems,” Brazilian Archives of Biology and Technology, vol. 60, no. 0, 2018. View at Publisher · View at Google Scholar