Optimization (Central Composite Design) and Validation of HPLC Method for Investigation of Emtricitabine Loaded Poly(lactic-co-glycolic acid) Nanoparticles: <i>In Vitro</i> Drug Release and <i>In Vivo</i> Pharmacokinetic Studies

<table>Comparison and effect of free emtricitabine and emtricitabine NPs on <svg height="15.65" id="M100" style="vertical-align:-3.39066pt" version="1.1" viewbox="0 0 60.150002 15.65" width="60.150002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>. Statistics: comparison based on <svg height="15.65" id="M101" style="vertical-align:-3.39066pt" version="1.1" viewbox="0 0 60.150002 15.65" width="60.150002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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q-4 29 -24 40.5t-73 15.5v28h238z" id="x55"></path></g><g transform="matrix(.017,-0,0,-.017,23.334,11.362)"><path d="M614 175l29 -10q-33 -109 -57 -154q-121 -26 -184 -26q-90 0 -160.5 29t-112.5 77t-63.5 105.5t-21.5 119.5q0 157 108 253t277 96q36 0 71.5 -5t69 -13.5t36.5 -8.5q15 -102 20 -150l-29 -8q-20 79 -66.5 114t-128.5 35q-119 0 -187.5 -86t-68.5 -207
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<g transform="matrix(.012,-0,0,-.012,34.638,15.45)"><path d="M241 635q53 0 94 -28.5t63.5 -76t33.5 -102.5t11 -116q0 -58 -11 -112.5t-34 -103.5t-63.5 -78.5t-94.5 -29.5t-95 28t-64.5 75t-34.5 102.5t-11 118.5q0 58 11.5 112.5t34.5 103t64.5 78t95.5 29.5zM238 602q-32 0 -55.5 -25t-35.5 -68t-17.5 -91t-5.5 -105
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</svg> by two-tailed unpaired <svg height="10.3875" id="M102" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 5.9250002 10.3875" width="5.9250002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,10.125)"><path d="M324 430l-26 -36l-112 -4l-55 -265q-13 -66 7 -66q13 0 44.5 20t50.5 40l17 -24q-38 -40 -85.5 -73.5t-87.5 -33.5q-50 0 -21 138l55 262h-80l-2 8l25 34h66l25 99l78 63l10 -9l-37 -153h128z" id="x1D461"></path></g>
</svg>-test with Welch’s correlation test ***<svg height="11.375" id="M103" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 66.875 11.375" width="66.875" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>.</table>

The Scientific World Journal

fig6

Figure 6

Figure 6: Optimization (Central Composite Design) and Validation of HPLC Method for Investigation of Emtricitabine Loaded Poly(lactic-co-glycolic acid) Nanoparticles: <i>In Vitro</i> Drug Release and <i>In Vivo</i> Pharmacokinetic Studies 

Figure 6 | Optimization (Central Composite Design) and Validation of HPLC Method for Investigation of Emtricitabine Loaded Poly(lactic-co-glycolic acid) Nanoparticles: In Vitro Drug Release and In Vivo Pharmacokinetic Studies 