Optimization (Central Composite Design) and Validation of HPLC Method for Investigation of Emtricitabine Loaded Poly(lactic-co-glycolic acid) Nanoparticles: In Vitro Drug Release and In Vivo Pharmacokinetic Studies

Table 3

System suitability parameters.

Parameter	Compound
	Emtricitabine	IS
Retention time (Rt)	4.39	6.58
Tailing factor (𝑇)	1.01	1.13
^†Injection repeatability (RSD)	0.752	0.623
^‡Resolution (𝑅𝑠)	—	7.32
Capacity factor (𝐾^′)	5.92	6.30
Theoretical plates (N)	4976	5318
Asymmetry	1.23	1.36

†RSD of peak areas of six consecutive injections at a concentration of 2.0 and 0.2 µg/mL of emtricitabine and IS, respectively.
^‡Resolution between emtricitabine and IS.