Optimization (Central Composite Design) and Validation of HPLC Method for Investigation of Emtricitabine Loaded Poly(lactic-co-glycolic acid) Nanoparticles: In Vitro Drug Release and In Vivo Pharmacokinetic Studies

Table 5

Stability of emtricitabinein rat plasma (𝑛=6).

Sample condition	Spiked concentration (μg/mL)	Mean determined concentration; (µg/mL)	Accuracy; (%)
Bench-top stability^♦	0.1	0.096	96.0
	0.8	0.783	97.87
	1.6	1.571	98.18
	2.0	1.944	97.20
Freeze-thaw stability^♣	0.1	0.099	99.00
	0.8	0.795	99.37
	1.6	1.582	98.87
	2.0	1.995	99.75
One-week stability^▲	0.1	0.093	93.00
	0.8	0.746	93.25
	1.6	1.517	94.81
	2.0	1.911	95.55

♦Exposed at ambient temperature (25°C) for 4 h.
^♣After three freeze-thaw cycles.
^▲Stored at −16°C.