The Scientific World Journal

Research Article

A Method for the Simultaneous Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Industrial Chicory Root Foodstuffs

Table 2

Authentication of CGA and STL in chicory flour by negative-ion UPLC-HRMS analysis.


Metabolites^a	Formula	M − H+	RDB	RT	M − H+	Precision (ppm)
Metabolites^a	Formula	theoretical	RDB	min	average ± SD	average ± SD

5-CQA		353.08726	8.5	1.85
DHLc	C₁₅H₁₈O₅	277.10760	7.5	2.07
Lc	C₁₅H₁₆O₅	275.09970	8.5	2.30
3,5-diCQA	C₂₅H₂₄O₁₂	515.11895	14.5	3.65
DHdLc	C₁₅H₁₈O₄	261.11260	7.5	4.04
dLc	C₁₅H₁₆O₄	259.09700	8.5	4.04
DHLp	C₂₃H₂₄O₇	411.14430	12.5	6.82
Lp	C₂₃H₂₂O₇	409.12870	13.5	6.91

5-CQA: 5-mono-O-caffeoylquinic acid; DHLc: 11(S),13-dihydrolactucin; Lc: lactucin; 3,5-diCQA: 3,5-di-O-caffeoylquinic acid; DHdLc: 11(S),13-dihydro-8-deoxylactucin; dLc: 8-deoxylactucin; DHLp: 11(S),13-dihydrolactucopicrin; Lp: lactucopicrin.
M − H+: molecular weight without one hydrogen.
RDB: ring double bond.
RT: retention times.
SD: standard deviations obtained by three repetitions.