A Method for the Simultaneous Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Industrial Chicory Root Foodstuffs

Table 3

Retention time (RT), limit of detection (LOD) and of quantitation (LOQ), linearity range, determination coefficient (), and regression equation of metabolites tested.

Metabolites^a	RT	LOD	LOQ	Linearity range		Regression equation
	avg ± SD	ng/mL	ng/mL	ng/mL
5-CQA		8.1	8.5	8.5–42,000	0.9993
DHLc		2	5.7	5.7–35,000	0.9930
Lc		6.4	13.7	13.7–70,000	0.9954
3,5-diCQA		9.3	66.5	66.5–62,000	0.9986
DHdLc		9.5	18.1	18.1–50,000	0.9954
dLc		4.1	11.4	11.4–10,000	0.9903
DHLp		2.5	3.3	3.3–2,000	0.9645
Lp		5.9	20.5	20.5–12,000	0.9910

5-CQA: 5-mono-O-caffeoylquinic acid; DHLc: 11(S),13-dihydrolactucin; Lc: lactucin; 3,5-diCQA: 3,5-di-O-caffeoylquinic acid; DHdLc: 11(S),13-dihydro-8-deoxylactucin; dLc: 8-deoxylactucin; DHLp: 11(S),13-dihydrolactucopicrin; Lp: lactucopicrin.