Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem's Delight Orchid with Higher Efficiency
Figure 4
Optimization of the Agrobacterium-mediated transformation based on transient gusA expression on VKD’s PLBs. (a) PLB size; (b) wounding; (c) concentration of acetosyringone; (d) cocultivation period; (e) Agrobacterium density; and (f) Immersion period. Results were analysed by one-way ANOVA and means were compared by Tukey’s test. Vertical bars represent ± SE of means of 6 replicates. Different letters indicate values which are significantly different .
