Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem's Delight Orchid with Higher Efficiency
Figure 5
Molecular analysis of transgenesintegration in putative transgenic plantlets and control PLBs. (a) Single band of 300 bp which was produced on lanes 2 and 3 confirmed the transfer of wwin1 and wwin2 genes (pr4 genes) into the PLBs (lane 1, marker; lane 2, putative transformant PLB (pW1B1); lane 3, putative transformant PLB (pW2KY); lane 4, untransformed PLB (control); Lane 5, A. tumefaciens (pW1B1); lane 6, A. tumefaciens (pW2KY)); (b) single band of 400 bp was produced on lanes 5 and 6 confirmed the transfer of nptII genes into the PLBs (lane 1, putative transformant PLB (pW1B1); lane 2, putative transformant PLB (pW2KY); lane 3, untransformed PLB (control); lane 4, marker).
