Research Article

White Mulberry (Morus alba) Foliage Methanolic Extract Can Alleviate Aeromonas hydrophila Infection in African Catfish (Clarias gariepinus)

Table 5

Experiment 2: hematological and biochemical indices in African catfish fed different amounts (2, 5 or 7 g/kg dry matter) of Morus alba foliage extract (MFE) with infection of fish with A. hydrophila (I) and compared to controls without or with a supplement of antibiotic oxytetracycline. (A) and without or with infection of fish with A. hydrophila (I).

ParametersDiets1
CNT2CNT2-ICNT2-I-AMFE-2-IMFE-5-IMFE-7-I

Red blood cells (106/μL)2.87 ± 0.06a2.45 ± 0.04b2.83 ± 0.03a2.52 ± 0.07b2.49 ± 0.05b2.79 ± 0.03a
White blood cells (105/μL)3.62 ± 0.07b4.05 ± 0.08a3.50 ± 0.12b3.98 ± 0.09a3.88 ± 0.11a3.54 ± 0.10b
Hemoglobin (g/dL)10.04 ± 0.65a8.65 ± 0.47b9.96 ± 0.30a8.45 ± 0.62b9.52 ± 0.51ab10.21 ± 0.75a
Hematocrit (%)43.2 ± 3.4a30.7 ± 3.9b45.0 ± 2.6a27.6 ± 3.3b37.3 ± 3.5ab38.7 ± 4.8a
Total protein (g/100 mL)46.6 ± 2.9ab32.2 ± 3.0c52.2 ± 2.4a31.0 ± 2.8c35.5 ± 2.5b48.3 ± 3.1a
Albumin (g/100 mL)22.7 ± 2.6a14.8 ± 3.0b25.9 ± 2.7a15.2 ± 2.2b17.2 ± 2.4b23.1 ± 3.2a
Globulin (g/100 mL)23.9 ± 3.2a17.4 ± 3.1b26.3 ± 2.1a15.8 ± 3.4b18.3 ± 2.6ab25.2 ± 3.0a

Means within the same row with different superscript letters are significantly different ().
1CNT2, control in Experiment 2; CNT2-I, fish infected with A. hydrophila and fed the control diet without any additive; CNT2-I-A, fish infected with A. hydrophila and fed the control diet supplemented with the antibiotic oxytetracycline (1 g/199 g DM), while MFE-2-I, MFE-5-I, and MFE-7-I were control diet plus 2, 5, or 7 g/kg Morus alba foliage extract, respectively, and infected with A.hydrophila. Values are the treatment means ± standard error.