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The Scientific World Journal
Volume 2014, Article ID 594579, 9 pages
http://dx.doi.org/10.1155/2014/594579
Research Article

Transcriptome Sequencing in a Tibetan Barley Landrace with High Resistance to Powdery Mildew

1Barley Improvement and Yak Breeding Key Laboratory, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, China
2College of Forestry, Sichuan Agricultural University, Yaan, Sichuan 625014, China

Received 20 October 2014; Revised 1 December 2014; Accepted 1 December 2014; Published 22 December 2014

Academic Editor: ChangHui Shen

Copyright © 2014 Xing-Quan Zeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hulless barley is an important cereal crop worldwide, especially in Tibet of China. However, this crop is usually susceptible to powdery mildew caused by Blumeria graminis f. sp. hordei. In this study, we aimed to understand the functions and pathways of genes involved in the disease resistance by transcriptome sequencing of a Tibetan barley landrace with high resistance to powdery mildew. A total of 831 significant differentially expressed genes were found in the infected seedlings, covering 19 functions. Either “cell,” “cell part,” and “extracellular region” in the cellular component category or “binding” and “catalytic” in the category of molecular function as well as “metabolic process” and “cellular process” in the biological process category together demonstrated that these functions may be involved in the resistance to powdery mildew of the hulless barley. In addition, 330 KEGG pathways were found using BLASTx with an E-value cut-off of <10−5. Among them, three pathways, namely, “photosynthesis,” “plant-pathogen interaction,” and “photosynthesis-antenna proteins” had significant matches in the database. Significant expressions of the three pathways were detected at 24 h, 48 h, and 96 h after infection, respectively. These results indicated a complex process of barley response to powdery mildew infection.