Activity of a Carboxyl-Terminal Truncated Form of Catechol 2,3-Dioxygenase from Planococcus sp. S5

Table 1

Strains and plasmids.

Strain or plasmid	Genotype and/or properties (reference); source
DH5C23OB61	E. coli 𝐷𝐻5𝛼[𝐹^− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17( 𝑟𝐾^− 𝑚𝐾^+), 𝜆–] harboring c23oB61 gene [22]; laboratory stock
E. coli BL21	[fhuA2 [lon] ompT gal [dcm] ΔhsdS]; New England Biolabs
BL21C23Owt	E. coli BL21 harboring c23owt gene [22]; laboratory stock
BL21C23OB61	E. coli BL21 harboring c23oB61 gene (this work)
pUC19 c23oB61	Amp^r, pUC19 derivative, c23oB61 encoding mutant form of catechol 2,3-dioxygenase (C103R, K289Stop) [22]
pET-22(b)	Amp^r; Novagen
pc23oB61	Amp^r, pET-22(b) derivative, c23oB61 encoding mutant form of catechol 2,3-dioxygenase (C103R, K289Stop) (this work)