Activity of a Carboxyl-Terminal Truncated Form of Catechol 2,3-Dioxygenase from Planococcus sp. S5

Table 2

Specific and relative activity of wild-type (wt) and mutant C23O dioxygenases using different catechols as the substrate.

Substrate	C23Owt			C23OB61
	Specific activity; (mU/mg)^a	Relative; activity^b %	Relative; activity^c %	Specific activity; (mU/mg)^a	Relative; activity^b %	Relative; activity^c %
Catechol	20.90±0.94^∗	100	100	16.45±0.83	78.70	100
3-Methylcatechol	1.28±0.55	100	6.12	2.33±1.09	182.03	14.16
4-Methylcatechol	21.30±1.56	100	101.91	30.98±3.74^∗	145.45	188.32
4-Chlorocatechol	9.38±2.48	100	44.88	16.75±1.76^∗	178.57	101.82

^∗𝑃<0.05. aOne unit of C23O activity was defined as the enzyme amount required generating 1 µmol of product per minute. The specific activity is defined in mU per milligram of protein.
^bExpressed as a percentage of the C23Owt-specific activity which is set as 100%.
^cExpressed as a percentage of the C23Os-specific activity toward catechol as a substrate which is set as 100%.
All data shown were expressed as mean ± standard deviation (𝑛=4).