Overexpression of the Synthetic Chimeric Native-T-phylloplanin-GFP Genes Optimized for Monocot and Dicot Plants Renders Enhanced Resistance to Blue Mold Disease in Tobacco (N. tabacum L.)
Figure 3
Cell wall targeted analysis of fused nat-T-phylloplanin-GFP in transgenic tobacco (Nicotiana tabacum cv. KY 14) lines. (a) The GFP concentration (expressed as μg GFP per mg protein) measured in leaf extracellular fluid (EF) of transgenic and wild-type plants. GFP was measured with the Turner Biosystems Luminometer by using the GFP-UV module. (b) Comparison of GFP concentration (μg GFP per mg protein) in total soluble protein (TP), extracellular fluid (EF), and postextracellular fluid (PEF) fractions measured in leaf samples of transgenic and wild-type plants. GFP was measured with the Turner Biosystems Luminometer by using the GFP-UV module. (c) Malate dehydrogenase (MDH) activity (expressed in units per mg protein) in total protein (TP), extracellular fluid (EF), and postextracellular fluid (PEF) fractions measured in leaf samples of transgenic and wild-type plants. Error bars indicate the standard deviation of readings from five different plants of each line (three readings per plant). W: wild-type KY14; GFP: pKM24KH-GFP transgenic plant expressing only GFP; T-phylloplanin-GFP transgenic lines: ibm8T1.1 to ibm8T1.4 and ibm10T2.1 to ibm10T2.2.
