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The Scientific World Journal
Volume 2014, Article ID 601845, 10 pages
http://dx.doi.org/10.1155/2014/601845
Research Article

Characterization of Developmental- and Stress-Mediated Expression of Cinnamoyl-CoA Reductase in Kenaf (Hibiscus cannabinus L.)

1School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea
2Department of Biosystems Machinery Engineering, Chungnam National University, Daejeon 305-764, Republic of Korea
3Department of Applied Biology, Chungnam National University, Daejeon 305-764, Republic of Korea
4Department of Crop Science, Chungnam National University, Daejeon 305-754, Republic of Korea
5Department of Bioenergy Science & Technology, Chonnam National University, Gwangju 500-757, Republic of Korea
6US Department of Agriculture, Agricultural Research Service, Invasive Insects Biocontrol & Behavior Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705, USA

Received 8 October 2013; Accepted 29 December 2013; Published 26 February 2014

Academic Editors: D. Caparros-Ruiz and H. Seitz

Copyright © 2014 Ritesh Ghosh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cinnamoyl-CoA reductase (CCR) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis. We have cloned a full length coding sequence of CCR from kenaf (Hibiscus cannabinus L.), which contains a 1,020-bp open reading frame (ORF), encoding 339 amino acids of 37.37 kDa, with an isoelectric point (pI) of 6.27 (JX524276, HcCCR2). BLAST result found that it has high homology with other plant CCR orthologs. Multiple alignment with other plant CCR sequences showed that it contains two highly conserved motifs: NAD(P) binding domain (VTGAGGFIASWMVKLLLEKGY) at N-terminal and probable catalytic domain (NWYCYGK). According to phylogenetic analysis, it was closely related to CCR sequences of Gossypium hirsutum (ACQ59094) and Populus trichocarpa (CAC07424). HcCCR2 showed ubiquitous expression in various kenaf tissues and the highest expression was detected in mature flower. HcCCR2 was expressed differentially in response to various stresses, and the highest expression was observed by drought and NaCl treatments.