The Scientific World Journal / 2014 / Article / Fig 3

Research Article

Use of Heavy Water (D2O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Figure 3

Thermostability of rp26 ELISA at 45°C. Duration of thermostability is indicated by arrow ( ) against strong positive serum (■), medium positive serum (●), and weak positive serum (▲). In 45°C, significant difference exists in 80% D2O versus 60% D2O, , 80% D2O versus H2O coating buffer, , at 2-month storage with respective serum strength.

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