Use of Heavy Water (D2O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection
Thermostability of rp26 ELISA at 45°C. Duration of thermostability is indicated by arrow () against strong positive serum (■), medium positive serum (●), and weak positive serum (▲). In 45°C, significant difference exists in 80% D2O versus 60% D2O, , 80% D2O versus H2O coating buffer, , at 2-month storage with respective serum strength.
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