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The Scientific World Journal
Volume 2014, Article ID 637183, 8 pages
Research Article

An Improved Quantitative Analysis Method for Plant Cortical Microtubules

1School of Life Sciences, Northwestern Polytechnical University, 127 Youyi xilu, Xi’an, Shaanxi 710072, China
2School of Science, Northwestern Polytechnical University, 127 Youyi xilu, Xi’an, Shaanxi 710072, China

Received 22 November 2013; Accepted 1 February 2014; Published 10 March 2014

Academic Editors: I. de la Serna, H. Fu, and J. Lin

Copyright © 2014 Yi Lu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The arrangement of plant cortical microtubules can reflect the physiological state of cells. However, little attention has been paid to the image quantitative analysis of plant cortical microtubules so far. In this paper, Bidimensional Empirical Mode Decomposition (BEMD) algorithm was applied in the image preprocessing of the original microtubule image. And then Intrinsic Mode Function 1 (IMF1) image obtained by decomposition was selected to do the texture analysis based on Grey-Level Cooccurrence Matrix (GLCM) algorithm. Meanwhile, in order to further verify its reliability, the proposed texture analysis method was utilized to distinguish different images of Arabidopsis microtubules. The results showed that the effect of BEMD algorithm on edge preserving accompanied with noise reduction was positive, and the geometrical characteristic of the texture was obvious. Four texture parameters extracted by GLCM perfectly reflected the different arrangements between the two images of cortical microtubules. In summary, the results indicate that this method is feasible and effective for the image quantitative analysis of plant cortical microtubules. It not only provides a new quantitative approach for the comprehensive study of the role played by microtubules in cell life activities but also supplies references for other similar studies.