Optimization of Processing Parameters for Extraction of Amylase Enzyme from Dragon (Hylocereus polyrhizus) Peel Using Response Surface Methodology
Table 2
-ratio and value for each independent variable effect in the polynomial response surface models.
Variables
Main effects
Quadratic effects
Interaction effects
Amylase activity (, U/mL)
value
0.033*
0.002*
0.000*
0.000*
0.045*
0.000*
0.001*
0.000*
0.023*
—
—
—
0.015*
0.011*
-ratio
7.84
19.20
39.69
86.06
5.76
14.44
17.64
50.41
10.17
—
—
—
12.69
13.96
Specific activity (, U)
value
0.040*
0.002*
0.013*
0.001*
0.033*
0.001*
0.006*
0.004*
—
—
—
0.001*
0.010*
—
-ratio
6.81
27.14
12.39
42.40
8.00
33.40
12.58
19.09
—
—
—
39.94
13.54
—
Temperature stability (, %)
value
—
0.000*
0.014*
0.000*
—
0.001*
0.008*
0.000*
—
—
—
—
0.017*
—
-ratio
—
28.70
8.09
28.17
—
19.89
9.78
20.16
—
—
—
—
7.44
—
pH stability (, %)
value
0.043*
0.005*
0.000*
0.015*
0.021*
0.001*
0.001*
0.006*
—
—
—
0.025*
—
0.044*
-ratio
3.34
11.42
24.90
7.95
7.09
12.60
18.92
5.29
—
—
—
5.05
—
1.78
Surfactant stability (, %)
value
0.040*
0.013*
0.015*
0.001*
0.011*
0.008*
0.001*
0.000*
—
—
—
0.004*
—
0.035*
-ratio
1.12
8.58
8.35
21.52
9.12
4.84
11.02
24.30
—
—
—
12.18
—
10.98
Storage stability (, %)
value
—
0.003*
0.036*
0.001*
—
0.030*
0.034*
0.014*
—
—
—
0.021*
—
0.000*
-ratio
—
17.05
5.76
21.06
—
6.65
6.22
15.52
—
—
—
7.84
—
37.94
, , , and : the main effect of temperature, time of mixing, pH of buffer and buffer to sample ratio, respectively. , , , and : the quadratic effect of effect of temperature, time of mixing, pH of buffer and buffer to sample ratio, respectively. : the interaction effect of temperature and time of mixing; : the interaction effect of temperature and pH of buffer, : the interaction effect of temperature and buffer to sample ratio; : interaction effect of time of mixing and pH of buffer; : interaction effect of pH of buffer and Buffer to sample ratio.
*Significant ().
