Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro

Table 2

Effect of Kinetin and IAA on mean weight and callus percentage of three O. viciifolia varieties: Golpaygan-181, Orumieh-1763, and Gorgan-1601 (leaf explants).

Kinetin (mg L^−1)	IAA (mg L^−1)	Weight (g)	Callus (%)	Colour	Texture	Embryo stage	Shoot/plant
0	0	0.046^c±0.002	26.67^bc±0.94	Green	CO	PE	—
0.5	0	0.337^b±0.028	55.00^b±1.45	Green	CO	GL	—
1	0	0.198^bc±0.019	86.67^a±2.35	Light G.	FR	GL	—
1.5	0	0.731^ab±0.034	97.50^a±3.58	Cream	FR	GL	—
2	0	0.292^b±0.016	52.50^b±1.98	Light G.	FR	PE	—
0	0.5	0.219^b±0.024	70.02^ab±2.14	Light G.	CO	CT	15
0.5	0.5	0.142^bc±0.09	68.33^ab±2.35	Light G.	FR	GL	—
1	0.5	0.639^ab±0.034	85.06^a±3.24	Cream	FR	GL	—
1.5	0.5	0.484^b±0.042	85.20^a±3.05	Light G.	CO	GL	—
2	0.5	0.121^bc±0.008	70.47^ab±2.45	Cream	FR	CT	—
0	1	0.179^bc±0.006	26.25^bc±0.98	Green	CO	PE	—
0.5	1	0.489^b±0.025	73.33^ab±2.45	Light G.	FR	GL	—
1	1	0.291^b±0.018	85.51^a±2.87	Light G.	CO	CT	—
1.5	1	0.163^bc±0.012	78.75^ab±2.34	Green	CO	CT	6
2	1	0.217^bc±0.015	68.33^ab±2.17	Light G.	FR	GL	—
0	1.5	0.174^bc±0.016	82.50^ab±3.78	Light G.	FR	CT	14
0.5	1.5	0.826^ab±0.057	100.0^a±3.45	Light G.	FR	CT	7
1	1.5	0.311^b±0.027	73.75^ab±3.15	Green	CO	CT	2
1.5	1.5	0.724^ab±0.065	96.25^a±3.54	Green	CO	GL	—
2	1.5	1.182^a±0.102	76.67^ab±2.97	Green	CO	GL	—
0	2	0.051^c±0.002	11.25^c±0.54	Green	CO	PE	18
0.5	2	1.015^a±0.098	100.0^a	Light G.	FR	CT	—
1	2	0.288^b±0.017	92.50^a±2.54	Green	CO	GL	—
1.5	2	1.351^a±0.114	95.00^a±2.58	Light G.	FR	CT	—
2	2	0.266^b±0.016	88.33^a±2.41	Green	CO	GL	—

The means of the populations with the same small letters were not significantly different as per Duncan’s multirange test at 𝑃<0.05.
CO: compact, FR: friable, PE: preembryo, GL: globular, CT: cotyledon, G: green.