Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro

Table 4

The responses of multiple shoots derived from encapsulated seeds on MS medium supplemented with different auxins and cytokinins concentrations.

MS + hormones (mg L^−1)		Observations (%)				Number
	BAP	Shoot	Callus	Necrosis	Root	Root/plant	Shoot/plant
Control		NR	86.47	12.24	NR	—	—
IBA
1	0.5	72.24±3.24	22.48±1.12	2.34±0.15	NR	—	18
2	0.5	48.76±1.57	33.37±1.57	16.66±1.10	3.23±0.24	1	12
0.5	—	NR	23.24±1.68	75.94±2.68	NR	—	—
1	—	NR	78.23±2.87	5.35±0.24	12.84±1.25	2	—
0.25	0.25	24.26±1.14	12.35±0.27	52.95±1.68	NR	—	14
2	—	NR	42.35±1.22	32.95±1.27	18.24±1.36	2	—
NAA
1	0.5	NR	NR	12.48±0.95	82.35±2.68	3	—
0.5	—	21.35±1.24	72.14±3.07	5.87±0.14	NR	—	7
0.25	—	NR	10.24±0.68	48.41±1.26	42.68±1.63	2	—
0.5	—	NR	28.35±1.34	61.84±2.65	10.24±1.15	—	—
2	—	NR	9.87±0.36	35.24±1.14	58.95±1.74	—	—
IAA
0.25	0.25	6.24±0.24	46.23±1.84	2.45±0.14	47.68±1.36	4	4
1	0.25	NR	66.24±2.16	32.54±1.36	NR	—	—
2	0.5	NR	75.62±3.27	12.87±1.08	NR	—	—
1	0.5	NR	72.32±3.16	15.84±1.24	NR	—	—
1	0.25	12.35±0.47	32.24±1.47	10.23±0.84	46.98±1.42	2	6
2	—	NR	58.62±1.88	42.32±2.15	NR	—	—
1	—	NR	42.35±1.65	48.95±2.06	NR	—	—
Half strength
1 mg/L IBA	—	NR	42.65±2.14	10.25±1.02	48.01±1.55	3	—
1 mg/L IAA	—	78.95±2.57	NR	12.52±1.24	10.65±0.84	1	11
1 mg/L NAA	—	NR	23.65±1.06	24.51±1.28	52.62±2.47	2	—

NR = no response was obtained.