Highly Effective Ex Vivo Gene Manipulation to Study Kidney Development Using Self-Complementary Adenoassociated Viruses
Diagram of the modified culture procedure and scAAV constructs used in this study. (a) Diagram of the modified culture procedure, in which kidney rudiments were incubated with transfection mixture or virus in 1.5 mL tube with low-serum medium for 4–6 hours at 4°C and then seeded and cultured on cover slip in 24-well plate. (b) Diagram of packaging vector containing AAV2-ITRs, CB promoter-driven EGFP transgene, and/or U6 promoter-driven shRNA. These vectors were packaged within different serotypes of capsids.