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The Scientific World Journal
Volume 2014, Article ID 692392, 7 pages
Research Article

Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant

1Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India
2Department of Applied Science, Kashi Institute of Technology, Varanasi, Uttar Pradesh 221307, India
3Department of Zoology, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India

Received 13 August 2013; Accepted 4 December 2013; Published 23 January 2014

Academic Editors: A. Menteşe and M. Oliveira

Copyright © 2014 Richa Upadhyay et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC50 of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of - transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant.