Four Different Methods Comparison for Extraction of Astaxanthin from Green Alga Haematococcus pluvialis

Table 2

Effect of different extraction methods on oil yield and total astaxanthin content (TAC) of extracts from H. Pluvialis cells.

Extraction technique^a	Solvent/raw material ratio (mL/g)	Extraction time (min)	Extraction oil yield (%, w/w)	TAC (mg/g-cell)
HCl-ACE	200	20	33.3±1.1	19.8±1.1
HEX-IPA	100	20	23.7±2.3	9.7±0.6
MET-ACE	400	20	24.3±0.6	13.8±0.4
Oil soy	8	120	26.0±1.0	0.9±0.1

aHCl: hydrochloric acid; ACE: acetone; HEX: hexane; IPA: isopropanol; MET: methanol; HCl-ACE: hydrochloric acid + acetone (5 : 5); Hex-IPA: hexane + isopropanol (6 : 4); MET-ACE: methanol + acetone (5 : 5); oil soy: extraction with soybean oil.