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The Scientific World Journal
Volume 2014, Article ID 703804, 8 pages
http://dx.doi.org/10.1155/2014/703804
Research Article

Myotubularin-Related Phosphatase 3 Promotes Growth of Colorectal Cancer Cells

1Department of Colorectal Surgery, Zhejiang Provincial People’s Hospital, Hangzhou, Zhejiang 310014, China
2Department of Gastroenterological Surgery, Zhejiang Provincial People’s Hospital, 158 Shangtang Road, Hangzhou, Zhejiang 310014, China

Received 18 March 2014; Revised 14 July 2014; Accepted 26 July 2014; Published 19 August 2014

Academic Editor: Toshimi Chiba

Copyright © 2014 Bo’an Zheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Due to changes in lifestyle, particularly changes in dietary habits, colorectal cancer (CRC) increased in recent years despite advances in treatment. Nearly one million new cases diagnosed worldwide and half a million deaths make CRC a leading cause of cancer mortality. In the present study, we aimed to investigate the role of myotubularin-related phosphatase 3 (MTMR3) in CRC cell growth via lentivirus-mediated small interfering RNA (siRNA) transduction in human colon cancer cell lines HCT116 and SW1116. The effect of MTMR3 knockdown on cell growth was evaluated by MTT, colony formation, and flow cytometry assays. The effect of MTMR3 knockdown on cell apoptosis was evaluated by flow cytometry with Annexin V/7-AAD double staining. The activation of apoptotic markers, Bad and PARP, was detected using Intracellular Signaling Array. Knockdown of MTMR3 resulted in a significant reduction in cell proliferation in both HCT116 and SW1116 cells. Moreover, knockdown of MTMR3 led to S phase cell cycle arrest. Furthermore, knockdown of MTMR3 induced cell apoptosis via phosphorylation of Bad and cleavage of PARP. These results indicate that MTMR3 may play an important role in the progression of CRC and suggest that siRNA mediated silencing of MTMR3 could be an effective tool in CRC treatment.