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The Scientific World Journal
Volume 2014, Article ID 704708, 9 pages
Research Article

Production of Silver Nanoparticles with Strong and Stable Antimicrobial Activity against Highly Pathogenic and Multidrug Resistant Bacteria

1Strategic Center for Diabetes Research, King Saud University, P.O. Box 18397, Riyadh 11415, Saudi Arabia
2The National Nanotechnology Center, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi Arabia
3University Diabetes Center, King Saud University, P.O. Box 18397, Riyadh 11415, Saudi Arabia

Received 12 March 2014; Revised 1 June 2014; Accepted 1 June 2014; Published 30 June 2014

Academic Editor: Kalimuthu Kalishwaralal

Copyright © 2014 Amr T. M. Saeb et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aims. To synthesize, characterize, and analyze antimicrobial activity of AgNPs of Escherichia hermannii (SHE), Citrobacter sedlakii (S11P), and Pseudomonas putida (S5). Methods. The synthesized AgNPs were examined using ultraviolet-visible spectroscopy (UV-vis) and, zeta potential, and the size and the morphology obtained from the three different isolates were also confirmed by TEM. Results. Among the three isolates tested, SHE showed the best antimicrobial activity due to the presence of small (4–12 nm) and stable (−22 mV) AgNPs. Stability of AgNPs was also investigated and found to be dependent on the nature of isolates. Conclusion. Produced AgNPs showed particle stability and antimicrobial efficacy up to 90 days of production. Our AgNPs exhibited greater antimicrobial activity compared with gentamicin against P. aeruginosa isolates and vancomycin against S. aureus and MRSA isolates at very low concentration (0.0002 mg per Microliters).