Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions

Figure 1

Experimental device developed under a continuous drip flow system and designed for dynamic biofilm formation under anaerobic conditions. Plastic container with fresh growth medium (a) connected to a plastic chamber containing the specimen (b). The flow was controlled to a velocity of 0.5 mL/min (c) and the waste collected on a second container (d). Stepwise scale-up strategy to reach a final concentration of 1.5 × 10⁸ cells/mL (e).