A Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cyprinid Herpesvirus 2 in Gibel Carp (Carassius auratus gibelio)
Figure 2
Optimization of the LAMP reaction conditions. (a) Reaction temperature: 1–6: 60°C, 61°C, 62°C, 63°C, 64°C, and 65°C, M: DL2000 DNA marker. (b) MgCl2 concentration: 1–6: 0 mM, 2 mM, 4 mM, 6 mM, 8 mM, and 10 mM MgCl2: M: DL2000 DNA marker.