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The Scientific World Journal
Volume 2014, Article ID 726190, 9 pages
Research Article

Effectiveness of Alternative Methods for Toothbrush Disinfection: An In Vitro Study

1Department of Dentomaxillofacial Radiology, Gazi University Faculty of Dentistry, 82 Sokak, No. 4, Emek, 06510 Ankara, Turkey
2Department of Medical Microbiology, Gazi University Faculty of Dentistry, 82 Sokak, No. 4, Emek, 06510 Ankara, Turkey

Received 17 March 2014; Accepted 4 May 2014; Published 25 May 2014

Academic Editor: Masahiro Yoneda

Copyright © 2014 Ilkay Peker et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. This study aimed to evaluate the effectiveness of alternative methods for toothbrush disinfection. Methods. Two-hundred eighty toothbrushes were included in the study. The toothbrushes were divided into 7 groups and were contaminated by standardized suspensions of Lactobacillus rhamnosus (L. rhamnosus), Streptococcus mutans (S. mutans), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli). The following disinfectants were tested: 1% sodium hypochlorite (NaOCl), 100% and 50% white vinegar, microwave (MW) oven, ultraviolet (UV) sanitizer, and mouth rinse-containing propolis (MCP). Data were analyzed with Kruskal Wallis and Dunn’s tests. Results. Statistically significant differences were found between different methods and control group for all tested bacteria. There were statistically significant differences between all test groups for all microorganisms. MW was the most effective for L. rhamnosus and 100% white vinegar was the most effective method for S. mutans and S. aureus. NaOCl was the most effective for E. coli. Conclusion. This study showed that 100% white vinegar was considered to be effective for tested microorganisms. Similarly, 1% NaOCl is cost-effective, easily accessible, and comparatively effective for toothbrush disinfection. Because these agents are nontoxic, cost-effective and easily accessible, they may be appropriate for household use.