Establishment of an Efficient In Vitro Regeneration Protocol for Rapid and Mass Propagation of Dendrobium chrysotoxum Lindl. Using Seed Culture

Figure 1

In vitro regeneration of Dendrobium chrysotoxum through seed culture. (a) Seed swelling indicating successful in vitro germination on medium enriched with 2.5 mg/L BAP and 0.8 mg/L IAA. (b) In vitro development of seedlings complete with healthy leaves and roots. (c) Intense callus formation in medium augmented with 2.5 mg/L BAP and 0.8 mg/L IBA. (d) Rapid leaf multiplication in medium supplemented with 2.0 mg/L KN and 0.5 mg/L NAA. (e) High in vitro root proliferation after seedlings transferred to new medium with 0.5 mg/L BAP and 3.0 mg/L IBA. (f) Hardening of in vitro raised healthy plantlets in basal liquid medium containing charcoal and brick pieces along with fine threads of coconut husks.

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