Establishment of an Efficient In Vitro Regeneration Protocol for Rapid and Mass Propagation of Dendrobium chrysotoxum Lindl. Using Seed Culture

Table 1

In vitro seed germination response of Dendrobium chrysotoxum on Mitra medium supplemented with different plant growth regulators.

BAP (mg/L)	KN (mg/L)	IAA (mg/L)	IBA (mg/L)	NAA (mg/L)	Activated charcoal (%)	Seed germination percentage	Germinated seeds developing into plantlets after 8 weeks (%)	Callus response***
0	0	0	0	0	0.4	82.4 ± 3.4^e	75.01 ± 5.1^d	+++
0.5		0.5			0.4	90.2 ± 2.7^d	82.35 ± 4.6^c	+++
1.8		1.0			0.4	93.3 ± 4.8^c	90.44 ± 6.2^b	+++
2.0		2.0			0.4	98.1 ± 3.9^b	91.63 ± 3.7^b	++++
2.5			0.8		0.4	96.1 ± 4.3^a	82.22 ± 4.3^b	+++++
	1.0		1.0		0.4	93.5 ± 2.7^a	87.05 ± 2.8^a	++++
	1.5		1.5		0.4	95.2 ± 3.9^a	92.66 ± 3.2^b	++
	2.0			0.5	0.4	95.1 ± 2.5^a	86.47 ± 5.3^a	++++
	3.0			1.5	0.4	96.4 ± 3.6^a	87.20 ± 4.8^a	++++

The results are based on 8 replicates per treatment in three repeated experiments. ± indicates the standard deviation values. Means followed by same letter are not significantly different at = 0.05.
***++: poor; +++: average; ++++: good; +++++: high rate.