Establishment of an Efficient In Vitro Regeneration Protocol for Rapid and Mass Propagation of Dendrobium chrysotoxum Lindl. Using Seed Culture

Table 2

Effect of different plant growth regulators on the in vitro leaf and root formation of Dendrobium chrysotoxum in 10 weeks.

BAP (mg/L)	KN (mg/L)	IAA (mg/L)	IBA (mg/L)	NAA (mg/L)	Activated charcoal (%)	Number of leaves/plantlets	Leaf length (cm)	Number of roots/plantlets	Root length (cm)
0	0	0	0	0	0.4	2.4 ± 0.7^c	0.3 ± 0.08^c	3.0 ± 1.3^g	0.4 ± 0.1^c
0.5		0.5			0.4	2.3 ± 0.5^c	0.5 ± 0.04^a	4.0 ± 1.8^f	1.2 ± 0.4^b
1.8		1.0			0.4	3.0 ± 0.9^d	0.5 ± 0.05^a	5.0 ± 1.0^e	1.2 ± 0.3^b
2.0		2.0			0.4	2.9 ± 1.0^d	0.4 ± 0.06^b	4.5 ± 1.4^d	1.1 ± 0.9^b
2.5			0.8		0.4	3.9 ± 1.1^a	0.5 ± 0.07^a	4.7 ± 1.3^b	1.2 ± 0.3^b
	1.0		1.0		0.4	3.9 ± 0.9^a	0.4 ± 0.09^b	5.2 ± 1.5^c	1.3 ± 0.4^a
	1.5		1.5		0.4	2.4 ± 0.8^c	0.4 ± 0.04^b	5.4 ± 1.2^c	1.4 ± 0.1^c
	2.0			0.5	0.4	4.0 ± 1.4^b	0.5 ± 0.08^a	4.8 ± 1.3^b	1.2 ± 0.1^b
	3.0			1.5	0.4	3.7 ± 1.2^a	0.5 ± 0.06^a	6.1 ± 1.4^a	1.3 ± 0.7^a

The results are based on 8 replicates per treatment in three repeated experiments. ± indicates the standard deviation values. Means followed by same letter are not significantly different at = 0.05.