Establishment of an Efficient In Vitro Regeneration Protocol for Rapid and Mass Propagation of Dendrobium chrysotoxum Lindl. Using Seed Culture
Table 2
Effect of different plant growth regulators on the in vitro leaf and root formation of Dendrobium chrysotoxum in 10 weeks.
BAP (mg/L)
KN (mg/L)
IAA (mg/L)
IBA (mg/L)
NAA (mg/L)
Activated charcoal (%)
Number of leaves/plantlets
Leaf length (cm)
Number of roots/plantlets
Root length (cm)
0
0
0
0
0
0.4
2.4 ± 0.7c
0.3 ± 0.08c
3.0 ± 1.3g
0.4 ± 0.1c
0.5
0.5
0.4
2.3 ± 0.5c
0.5 ± 0.04a
4.0 ± 1.8f
1.2 ± 0.4b
1.8
1.0
0.4
3.0 ± 0.9d
0.5 ± 0.05a
5.0 ± 1.0e
1.2 ± 0.3b
2.0
2.0
0.4
2.9 ± 1.0d
0.4 ± 0.06b
4.5 ± 1.4d
1.1 ± 0.9b
2.5
0.8
0.4
3.9 ± 1.1a
0.5 ± 0.07a
4.7 ± 1.3b
1.2 ± 0.3b
1.0
1.0
0.4
3.9 ± 0.9a
0.4 ± 0.09b
5.2 ± 1.5c
1.3 ± 0.4a
1.5
1.5
0.4
2.4 ± 0.8c
0.4 ± 0.04b
5.4 ± 1.2c
1.4 ± 0.1c
2.0
0.5
0.4
4.0 ± 1.4b
0.5 ± 0.08a
4.8 ± 1.3b
1.2 ± 0.1b
3.0
1.5
0.4
3.7 ± 1.2a
0.5 ± 0.06a
6.1 ± 1.4a
1.3 ± 0.7a
The results are based on 8 replicates per treatment in three repeated experiments. ± indicates the standard deviation values. Means followed by same letter are not significantly different at = 0.05.