Establishment of an Efficient In Vitro Regeneration Protocol for Rapid and Mass Propagation of Dendrobium chrysotoxum Lindl. Using Seed Culture
Table 3
Effect of different plant growth regulators on the in vitro leaf and root formation of Dendrobium chrysotoxum in 3 weeks after first subculture.
BAP (mg/L)
KN (mg/L)
IAA (mg/L)
IBA (mg/L)
NAA (mg/L)
Activated charcoal (%)
Number of leaves/plantlets
Leaf length (cm)
Number of roots/plantlets
Root length (cm)
0
0
0
0
0
0.4
3.8 ± 0.8d
0.4 ± 0.2d
5.7 ± 2.9b
1.2 ± 1.0e
0.5
0.5
0.4
3.2 ± 1.0c
0.8 ± 0.2b
6.1 ± 2.4c
3.2 ± 0.1d
1.8
1.0
0.4
3.3 ± 0.8c
0.7 ± 0.6b
6.1 ± 2.6c
3.1 ± 0.9d
2.0
2.0
0.4
3.2 ± 0.8c
0.7 ± 0.4b
6.7 ± 0.5e
3.0 ± 1.2d
2.5
0.8
0.4
4.1 ± 1.1e
1.0 ± 0.3a
5.3 ± 0.9d
2.0 ± 0.9c
1.0
1.0
0.4
3.9 ± 1.6d
0.6 ± 0.2c
5.6 ± 2.1b
3.6 ± 0.8a
1.5
1.5
0.4
3.1 ± 0.8c
0.7 ± 0.2b
6.1 ± 1.9c
3.8 ± 0.6a
2.0
0.5
0.4
5.0 ± 0.9b
1.1 ± 0.2a
5.8 ± 1.1b
2.8 ± 1.2b
3.0
1.5
0.4
4.8 ± 0.9a
0.9 ± 0.6a
7.7 ± 0.8a
3.7 ± 1.4a
The results are based on 8 replicates per treatment in three repeated experiments. ± indicates the standard deviation values. Means followed by same letter are not significantly different at = 0.05. First subculture was performed at 10 weeks from date of inoculation.