Establishment of an Efficient In Vitro Regeneration Protocol for Rapid and Mass Propagation of Dendrobium chrysotoxum Lindl. Using Seed Culture

Table 3

Effect of different plant growth regulators on the in vitro leaf and root formation of Dendrobium chrysotoxum in 3 weeks after first subculture.

BAP (mg/L)	KN (mg/L)	IAA (mg/L)	IBA (mg/L)	NAA (mg/L)	Activated charcoal (%)	Number of leaves/plantlets	Leaf length (cm)	Number of roots/plantlets	Root length (cm)
0	0	0	0	0	0.4	3.8 ± 0.8^d	0.4 ± 0.2^d	5.7 ± 2.9^b	1.2 ± 1.0^e
0.5		0.5			0.4	3.2 ± 1.0^c	0.8 ± 0.2^b	6.1 ± 2.4^c	3.2 ± 0.1^d
1.8		1.0			0.4	3.3 ± 0.8^c	0.7 ± 0.6^b	6.1 ± 2.6^c	3.1 ± 0.9^d
2.0		2.0			0.4	3.2 ± 0.8^c	0.7 ± 0.4^b	6.7 ± 0.5^e	3.0 ± 1.2^d
2.5			0.8		0.4	4.1 ± 1.1^e	1.0 ± 0.3^a	5.3 ± 0.9^d	2.0 ± 0.9^c
	1.0		1.0		0.4	3.9 ± 1.6^d	0.6 ± 0.2^c	5.6 ± 2.1^b	3.6 ± 0.8^a
	1.5		1.5		0.4	3.1 ± 0.8^c	0.7 ± 0.2^b	6.1 ± 1.9^c	3.8 ± 0.6^a
	2.0			0.5	0.4	5.0 ± 0.9^b	1.1 ± 0.2^a	5.8 ± 1.1^b	2.8 ± 1.2^b
	3.0			1.5	0.4	4.8 ± 0.9^a	0.9 ± 0.6^a	7.7 ± 0.8^a	3.7 ± 1.4^a

The results are based on 8 replicates per treatment in three repeated experiments. ± indicates the standard deviation values. Means followed by same letter are not significantly different at = 0.05.
First subculture was performed at 10 weeks from date of inoculation.