Establishment of an Efficient In Vitro Regeneration Protocol for Rapid and Mass Propagation of Dendrobium chrysotoxum Lindl. Using Seed Culture

Table 4

Effect of different plant growth regulators on leaf and root formation after seedlings transferred from initial to new plant growth regulator combinations in 2 weeks after 4th subculture.

Initial PGR combinations with 0.4% AC		New PGR combinations with 0.4% AC	Number of leaves/plantlets	Leaf length (cm)	Number of roots/plantlets	Root length (cm)
0.5 mg/L IAA; + 0.5 mg/L BAP	→	0.8 mg/L IBA; + 2.5 mg/L BAP	3.8 ± 1.2^e	1.4 ± 0.9^c	6.8 ± 1.2^e	2.1 ± 1.1^e
2.5 mg/L BAP; + 0.8 mg/L IBA	→	0.5 mg/L BAP; + 3.0 mg/L IBA	3.4 ± 0.4^b	1.3 ± 0.4^d	13.2 ± 1.3^d	4.4 ± 1.7^d
	→	3.5 mg/L BAP; + 0.5 mg/L IBA	5.7 ± 1.0^d	2.5 ± 0.7^a	7.5 ± 1.8^c	2.5 ± 0.8^b
1.0 mg/L KN; + 1.0 mg/L IBA	→	4.5 mg/L NAA	3.4 ± 0.2^b	1.5 ± 0.6^c	11.1 ± 2.1^b	4.6 ± 0.4^c
2.0 mg/L KN; + 0.5 mg/LNAA	→	3.5 mg/L KN	4.1 ± 0.3^c	2.1 ± 0.3^b	7.3 ± 1.2^c	1.6 ± 0.7^a
3.0 mg/L KN; + 1.5 mg/L NAA	→	2.5 mg/L KN; + 2.5 mg/L NAA	3.4 ± 0.9^b	2.4 ± 0.4^a	12.0 ± 1.2^b	2.6 ± 1.4^b
	→	3.5 mg/L KN	4.2 ± 0.7^a	2.3 ± 0.6^a	9.0 ± 1.6^a	1.6 ± 0.8^a

The results are based on 8 replicates per treatment in three repeated experiments. ± indicates the standard deviation values. Means followed by same letter are not significantly different at = 0.05.
Fourth subculture was performed at 19 weeks from date of inoculation.