Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant
Figure 2
Effects of BAP and NAA on shoot formation from the immature explants (yellow plumule) of N. nucifera after 24 weeks in culture on MS medium. Yellow plumule cultured on MS solid media supplemented with 0.5 mg/L BAP and 1.5 mg/L NAA. The cultures were maintained at °C with 16 hours of light and 8 hours of dark, with 1000 lux intensity of light for 24 weeks. (a) Yellow pod with nine immature seeds. (b) Two hinged shoots (explants) on. (c) First subculture after 2 weeks cultured on solid MS basal (control). (d) Elongation of first shoot. (e) Two rolled shoots after 3 weeks. (f) Formation of layered shoots after 4 weeks. (g) Elongation of shoots after 6 weeks. (h) Formation of first unrolled leaf after 8 weeks. (i) Layered multiple shoots (vertical position) with formation of primary roots after 12 weeks.
