Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant

Table 1

The effect of double-layered media on the regeneration of shoots. Cultures were maintained at 25±1°C with 16 hours of light and 8 hours of dark, with 1000 lux intensity of light for 4 weeks.

Ratio liquid : solid (mm)	Number of shoots per explants (Mean ± SE)	Observations
0 : 1 (control)	9.10±0.51	Yellow shoots turned green and elongated. Primary root formation
1 : 1	15.67±0.09	Shoots elongated
2 : 1	16.67±0.23	Layered shoots. Primary root formation
3 : 1	10.33±0.23	Shoots turned brown

∗Double-layered medium (yellow shoot): combination of solid MS + 0.5 mg/L BAP + 1.5 mg/L NAA and liquid MS basal.
Each value represents the mean of thirty replicates. Mean ± standard error (SE), replicates (𝑛=30).