Characterization of Affinity-Purified Isoforms of Acinetobacter calcoaceticus Y1 Glutathione Transferases
Figure 1
(a) SDS-PAGE of purified GSTs from Acinetobacter calcoaceticus Y1. Lane 1 shows the benchmark standard marker (Invitrogen). Lane 2 shows the presence of single band GST with MW estimated at 23 kDa (10 μg). (b) 2-D gel electrophoresis (IPG 3–10) of purified GSTs resolved into two isoforms (30 μg). (c) Isoelectric-focusing of the purified GST (10 μg). The calculated pIs are 4.5 and 6.2 for GST1 and GST2, respectively. SERVA IEF marker (Invitrogen) was used for pI estimation. Gels were stained with colloidal coomassie blue (a and b) and silver (c).
