Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi

Table 1

Primers used to amplify the complete rDNA sequence of Paramphistomum cervi. The upper and lower sequences are forward (F) and reverse (R) for each primer, respectively.

Name of primer	Amplification regions	Primer sequence (5′-3′)	Annealing temperature (°C)	Length
P1	18S	F: TCTGTGATGACTCTGGAT	53.7	1,596 bp
		R: ACCATTCAATCGGTAGTA
P2	18S-28S	F: CACCGCCCGTCGCTACTACC	55.2	1,303 bp
		R: TACTTTTCAACTTTCCCTCA
P3	28S-1	F: TAGGCAATGTGGTGTT	54.7	1,156 bp
		R: TTGCACGTCAGAATCGCT
P4	28S-2	F: CGGAGACGGCGGCTTGTTGTG	57.1	1,608 bp
		R: GGCTGTTCACCTTGGAGA
P5	28S-3	F: ACAGAGACGGGGTGCCTG	51.7	1,390 bp
		R: AAAATCAAAATCAAGTAA
P6	28S-18S (IGS)	F: TACCACCACCGTCATTGTTTCTTTG	55.7	1,742 bp
		R: AAGTTATCCAGAGTCATCACAGAGT