Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava

Table 2

Soluble protein content, antioxidant enzyme activities (SOD, GPx and CAT), and glutathione, and total thiol content in A. flava.

Aesculus flava samples	Proteins; (mg/g)	SOD; (U/mg protein)	GPx; (nmol/mg protein)	CAT; (nmol/mg protein)	GSH; (μmol/mg protein)	TSH; (μmol/mg protein)
In vitro androgenic embryos in cotyledonary stage	1.0^a	1024^a	1515^a	31.6^a	2.7^a	2.7^a
In vitro androgenic embryos in globular stage	2.1^b	333.8^b	1493^a	14.3^b	1.0^b	1.0^b
Leaves in vivo (control)	0.9^a	1586.0^c	36.9^b	15.3^b	4.1^c	4.1^c

Values in rows marked with different letters (a, b, c, and d) were significantly different according to Duncan 𝑡-test 𝑃<0.05. For each parameter, experiments and measurements were also recorded in triplicate; SOD: superoxide dismutase; GPx: glutathione peroxidase; CAT: catalase; GSH: reduced glutathione; TSH: total thiols.