Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava

Table 4

Soluble protein content, antioxidant enzyme activities (SOD, GPx and CAT), and glutathione, and total thiol content in A. hippocastanum.

Aesculus hippocastanum organs	Proteins; (mg/g)	SOD; (U/mg protein)	GPx; (nmol/mg protein)	CAT; (nmol/mg protein)	GSH; (μmol/mg protein)	TSH; (μmol/mg protein)
In vitro androgenic embryos in cotyledonary stage	2.2^b	500.5^b	1290^b	11.9^b	0.9^b	0.9^b
Leaves in vivo (control)	0.6^c	3197^c	55.9^c	30.9^a	3.4^c	4.1^c
Seeds in vivo (control)	8.4^d	123.8^d	327.7^d	47.8^c	0.7^b	0.5^b

Values in rows marked with different letters (a, b, c, and d) were significantly different according to Duncan 𝑡-test 𝑃<0.05. For each parameter, experiments and measurements were also recorded in triplicate; SOD: superoxide dismutase; GPx: glutathione peroxidase; CAT: catalase; GSH: reduced glutathione; TSH: total thiols.