Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion of Arabidopsis Cellulose Synthase-Like A2 Inserted into Golgi Membrane

Figure 1

Subcellular localization of AtCslA2-GFP. AtCslA2-GFP transiently expressed in tobacco leaf protoplasts labels ER and Golgi stacks (a). Brefeldin A determines the aggregation of the Golgi and the redistribution of the fluorescence in the ER (b–d). AtCslA2-GFP colocalises with the Golgi marker ST52-mRFP (e–g). Golgi stacks are the only fluorescent compartment labelled after 2 h of cycloheximide treatment (h). Scale bar: 20 μm.

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