Molecular Divergence and Species Delimitation of the Cultivated Oyster Mushrooms: Integration of IGS1 and ITS

Table 2

List of designed and referenced PCR primers used in this study.

Primer ID	Type	Sequence	𝑇𝑚	GC%
LR12R	Forward	5′-CTGAACGCCTCTAAGTCAGAA-3′	53°C	—
LR13R	Forward	5′-GCATTGTTGTTCCGATG-3′	53°C	—
5SRNA	Reverse	5′-ATCAGACGGGATGCGGT-3′	53°C	—
ITS1	Forward	5′-TCCGTAGGTGAACCTGCGG-3′	55°C	—
ITS1f	Forward	5′-TCCTCCGCTTATTGATATGC-3′	57°C	—
ITS4	Reverse	5′-CTTGGTCATTTAGAGGAAGTAA-3′	55°C	—
ITS4B	Reverse	5′-CAGGAGACTTGTACACGGTCCAG-3′	57°C	—
IGS1-UM4	Forward	5′-AGTAAACTGACTTCAATTTCCGAGC-3′	55°C	40
IGS1-UM5	Reverse	5′-ATCCGCTGAGGTTAAGCCCT-3′	55°C	55
ITS1-UM2	Forward	5′-TAACAAGGTTTCCGTAGGTG-3′	55°C	45
ITS2-UM2	Reverse	5′-CTTAAGTTCAGCGGGTAGTC-3′	55°C	50