Blocked Autophagy by miR-101 Enhances Osteosarcoma Cell Chemosensitivity In Vitro

Figure 4

MiR-101 inhibits conversion of LC3-I to LC3-II and inhibits Atg 4 expression. (a) Western blotting results of Dox-treated U-2 OS cells simultaneously with miR control or miR-101 mimics transfection. (b) The conversion of LC3-I to LC3-II after miR-101 mimics or miR control transfection; (c) and (d) the percentage of Atg 5 or At 4 expression to GAPDH after miR-101 or miR control transfection (^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001). All results were from three independently performed experiments.

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