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The Scientific World Journal
Volume 2014, Article ID 810587, 6 pages
http://dx.doi.org/10.1155/2014/810587
Research Article

Clinicopathological and Molecular Findings in a Case of Canine Anaplasma phagocytophilum Infection in Northern Italy

1Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, Italy
2“Dr Nicola Mengoli” Veterinary Clinic, Via Di Mezzo 51, 40060 Toscanella di Dozza, Italy
3Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy

Received 8 April 2014; Accepted 22 May 2014; Published 5 June 2014

Academic Editor: Hiromi Nishida

Copyright © 2014 Francesco Dondi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. Z. Woldehiwet, “The natural history of Anaplasma phagocytophilum,” Veterinary Parasitology, vol. 167, no. 2–4, pp. 108–122, 2010. View at Publisher · View at Google Scholar · View at Scopus
  2. J. S. Dumler, A. F. Barbet, C. P. J. Bekker et al., “Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and “HGE agent” as subjective synonyms of Ehrlichia phagocytophila,” International Journal of Systematic and Evolutionary Microbiology, vol. 51, no. 6, pp. 2145–2165, 2001. View at Google Scholar · View at Scopus
  3. Y. O. Sanogo, P. Parola, S. Shpynov et al., “Genetic diversity of bacterial agents detected in ticks removed from asymptomatic patients in Northeastern Italy,” Annals of the New York Academy of Sciences, vol. 990, pp. 182–190, 2003. View at Google Scholar · View at Scopus
  4. S. Aureli, J. E. Foley, R. Galuppi, D. Rejmanek, C. Bonoli, and M. P. Tampieri, “Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy,” Veterinaria Italiana, vol. 48, no. 4, pp. 413–423, 2012. View at Google Scholar · View at Scopus
  5. G. Carpi, L. Bertolotti, E. Pecchioli, F. Cagnacci, and A. Rizzoli, “Anaplasma phagocytophilum groEL gene heterogeneity in Ixodes ricinus larvae feeding on roe deer in Northeastern Italy,” Vector-Borne and Zoonotic Diseases, vol. 9, no. 2, pp. 179–184, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. R. Zobba, A. G. Anfossi, M. L. P. Parpaglia et al., “Molecular investigation and phylogeny of Anaplasma spp. in Mediterranean ruminants reveal the presence of neutrophil-tropic strains closely related to A. platys,” Applied and Environmental Microbiology, vol. 80, no. 1, pp. 271–280, 2014. View at Publisher · View at Google Scholar
  7. A. Alberti, M. F. Addis, O. Sparagano et al., “Anaplasma phagocytophilum, Sardinia, Italy,” Emerging Infectious Diseases, vol. 11, no. 8, pp. 1322–1324, 2005. View at Google Scholar · View at Scopus
  8. A. Alberti, R. Zobba, B. Chessa et al., “Equine and canine Anaplasma phagocytophilum strains isolated on the island of Sardinia (Italy) are phylogenetically related to pathogenic strains from the United States,” Applied and Environmental Microbiology, vol. 71, no. 10, pp. 6418–6422, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. M. Ruscio and M. Cinco, “Human granulocytic ehrlichiosis in Italy: first report on two confirmed cases,” Annals of the New York Academy of Sciences, vol. 990, pp. 350–352, 2003. View at Google Scholar · View at Scopus
  10. A. M. Clark, G. F. Hopkins, and I. A. MacLean, “Tick-borne fever in dogs,” The Veterinary Record, vol. 139, no. 11, p. 268, 1996. View at Google Scholar · View at Scopus
  11. F. Gentilini, D. Mancini, F. Dondi et al., “Validation of a human immunoturbidimetric assay for measuring canine C-reactive protein,” Veterinary Clinical Pathology, vol. 34, supplement 318, 2005. View at Google Scholar
  12. F. Gentilini, F. Dondi, C. Mastrorilli et al., “Validation of a human immunoturbidimetric assay to measure canine albumin in urine and cerebrospinal fluid,” Journal of Veterinary Diagnostic Investigation, vol. 17, no. 2, pp. 179–183, 2005. View at Google Scholar · View at Scopus
  13. R. M. Barber, Q. Li, P. P. Diniz et al., “Evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for Ehrlichia, Anaplasma, spotted fever group Rickettsia, Bartonella, and Borrelia species in canine neurological diseases (109 cases),” Journal of Veterinary Internal Medicine, vol. 24, no. 2, pp. 372–378, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. J. D. Thompson, D. G. Higgins, and T. J. Gibson, “CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice,” Nucleic Acids Research, vol. 22, no. 22, pp. 4673–4680, 1994. View at Google Scholar · View at Scopus
  15. K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar, “MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods,” Molecular Biology and Evolution, vol. 28, no. 10, pp. 2731–2739, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. E. Spada, D. Proverbio, P. Galluzzo et al., “Molecular study on selected vector-borne infections in urban stray colony cats in Northern Italy,” Journal of Feline Medicine Surgery, 2013. View at Publisher · View at Google Scholar
  17. M. Canelas Domingos, M. Trotta, A. Briend-Marchal, and C. Medaille, “Anaplasmosis in two dogs in France and molecular and phylogenetic characterization of Anaplasma phagocytophilum,” Veterinary Clinical Pathology, vol. 40, no. 2, pp. 215–221, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. N. Tozon, M. Petrovec, and T. Avšič-Županc, “Clinical and laboratory features of the first detected cases of A. phagocytophila infections in dogs from Slovenia,” Annals of the New York Academy of Sciences, vol. 990, pp. 424–428, 2003. View at Google Scholar · View at Scopus
  19. A. E. Egenvall, A. A. Hedhammar, and A. I. Bjöersdorff, “Clinical features and serology of 14 dogs affected by granulocytic ehrlichiosis in Sweden,” The Veterinary Record, vol. 140, no. 9, pp. 222–226, 1997. View at Google Scholar · View at Scopus
  20. L. Manna, A. Alberti, L. M. Pavone, A. Scibelli, N. Staiano, and A. E. Gravino, “First molecular characterization of a granulocytic Ehrlichia strain isolated from a dog in South Italy,” Veterinary Journal, vol. 167, no. 3, pp. 224–227, 2004. View at Publisher · View at Google Scholar · View at Scopus
  21. U. Ravnik, N. Tozon, K. S. Smrdel, and T. A. Zupanc, “Anaplasmosis in dogs: the relation of haematological, biochemical and clinical alterations to antibody titre and PCR confirmed infection,” Veterinary Microbiology, vol. 149, no. 1-2, pp. 172–176, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. D. Rejmanek, G. Bradburd, and J. Foley, “Molecular characterization reveals distinct genospecies of Anaplasma phagocytophilum from diverse North American hosts,” Journal of Medical Microbiology, vol. 61, no. 2, pp. 204–212, 2012. View at Publisher · View at Google Scholar · View at Scopus
  23. G. Maioli, D. Pistone, P. Bonilauri et al., “Ethiological agents of rickettsiosis and anaplasmosis in ticks collected in Emilia-Romagna region (Italy) during 2008 and 2009,” Experimental and Applied Acarology, vol. 57, no. 2, pp. 199–208, 2012. View at Publisher · View at Google Scholar · View at Scopus