The Connection between the Toxicity of Anthracyclines and Their Ability to Modulate the P-Glycoprotein-Mediated Transport in A549, HepG2, and MCF-7 Cells

Table 1

Flow cytometry analysis of P-gp functional activity with R123 or JC-1 in A549, HepG2, and MCF-7 cells.

Sample	Intracellular accumulation of JC-1 and R123 (%)
	A549	HepG2	MCF-7
R123	100.0±5.1	100.0±7.2	100.0±6.2
R123 + V	70.6±7.2	124.89±3.7^∗	110.8±3.9
R123 + DOX	129.7±3.6^∗	126.88±9.2^∗	148.2±8.3^∗+
R123 + ACL	111.2±5.9	157.29±6.5^∗+	137.7±11.7^∗+
JC-1	100.0±2.9	100.0±4.7	100.0±8.6
JC-1 + V	85.2±5.6	212.41±5.2^∗	161.2±8.9^∗
JC-1 + DOX	92±5.5	250±2.9^∗+	386.8±13.3^∗+
JC-1 + ACL	86.9±13.9	212.59±7.0^∗+	158.3±14.6^∗

Results represent means ± SD of 4 independent experiments. *𝑃<0.05, significant differences between P-gp functional activity calculated from influx of R123 or JC-1 and R123 or JC-1 plus Verapamil and from R123 or JC-1 plus ACL or DOX after a 30-minute period of time monitoring the transport of fluorescence probes to the cells. ⁺𝑃<0.05, significant differences between positive control samples (cells preincubated with Verapamil) and cells treated with DOX or ACL. P-gp functional activity was evaluated based on the basis of fluorescence ratios of R123 or JC-1 (Figure 4) and calculated for each tested probe. The final results were presented as percentage values.