Molten Globule-Like Partially Folded State of Bacillus licheniformis α-Amylase at Low pH Induced by 1,1,1,3,3,3-Hexafluoroisopropanol
Figure 2
Far-UV (a) and near-UV (b) CD spectra of different conformational states of BLA, as obtained at 25°C using a protein concentration and cuvette path length of 1.7 μM; 1 mm and 3.4 μM; 10 mm for far-UV and near-UV CD spectral measurements, respectively. Native state (—), acid-denatured state (···), and HFIP-induced state (- - -).
