Molten Globule-Like Partially Folded State of Bacillus licheniformis α-Amylase at Low pH Induced by 1,1,1,3,3,3-Hexafluoroisopropanol
Figure 3
Intrinsic fluorescence (a) and ANS fluorescence (b) spectra of different conformational states of BLA, as obtained at 25°C using a protein concentration of 0.1 μM and 0.6 μM for intrinsic fluorescence and ANS fluorescence measurements, respectively. Native state (—), acid-denatured state (···), and HFIP-induced state (- - -).
