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The Scientific World Journal
Volume 2014, Article ID 839548, 6 pages
http://dx.doi.org/10.1155/2014/839548
Research Article

Histochemical Investigation and Kinds of Alkaloids in Leaves of Different Developmental Stages in Thymus quinquecostatus

1College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China
2Central Laboratory, Qingdao Agricultural University, Qingdao 266109, China
3University Key Laboratory of Plant Biotechnology, Shandong, Qingdao 266109, China

Received 5 April 2014; Revised 5 June 2014; Accepted 16 June 2014; Published 1 July 2014

Academic Editor: Karim Allaf

Copyright © 2014 Haiting Jing et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Thymus quinquecostatus, with more medical value, is a kind of wild plants. In order to exploit and utilize this plant, we studied the species and locations of alkaloids in its leaves. In this paper, histochemical study of leaves at different developing stages was taken to localize the alkaloids. Meanwhile, the kinds and content of alkaloids in leaves were identified using GC-MS technique. It was found that there were two kinds of glandular trichomes, namely, peltate trichomes and capitate trichomes, on the surface of leaves, and their secretory cells could secrete alkaloids. Results showed that trichomes could secrete alkaloids as soon as the first pair of leaves formed, and there were altogether 18 kinds of alkaloids identified by GC-MS. Nearly all of these alkaloids of leaves at different developing stages were distinct from each other, except one, 3-methoxy-a-methyl-benzeneethanamine, persists at different developing stages with high concentration.