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The Scientific World Journal
Volume 2014, Article ID 840863, 6 pages
http://dx.doi.org/10.1155/2014/840863
Research Article

Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by “SLRP”

1Department of Protein Research, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, New Borg Al-Arab, P.O. Box. 21934, Alexandria, Egypt
2Biotechnology Department, Institute of Graduate Studies & Research, Alexandria University, 163 El Horreya Avenu, P.O. Box 832, Alexandria 21526, Egypt
3Zoonoses Research Unit, Faculty of Veterinary Medicine, Alqadisya University, Alqadisya, Iraq
4Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

Received 6 January 2014; Accepted 25 February 2014; Published 19 March 2014

Academic Editors: L. Pochini and P. Poltronieri

Copyright © 2014 Amara A. Amro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Recently, bacterial ghosts (BGs) were prepared using a protocol based on critical chemical concentrations. It has been given the name “sponge like” (SL) protocol and used in its reduced form “sponge like reduced protocol” (SLRP). While specific antibody for Salmonella is available on the market under the commercial names (of some kits) such as Febrile Antigen Kit (N.S. BIO-TEC), we used the described Kit to investigate the validity of the SLRP. In this study, using SLRP we succeeded to prepare STGs with correct surface antigens could interact with their specific antibodies. Additionally the study has included oral vaccination with STGs with challenge test. The rats serums have been evaluated against both of the O and H antigens. The antigen-antibody interaction (agglutination) results of both the SLRP and the animal experiments prove that we have correct STGs able to immunize the rats against viable Salmonella. STGs could be used as vaccine and as adjuvant and in the antibodies and in the diagnostic kits production. This study is an additional step for the establishment of correct BGs for immunological purposes.